Gene Silencing at the Nuclear Periphery Essay Example | Topics and Well Written Essays - 1000 words

Gene Silencing at the Nuclear Periphery - Essay ExampleSome epigenetic abnormalities have been set in human tumors, offering tremendous scope for epigenetic modulation as part of cancer chemotherapy. (TY Kim, YJ Bang, KD Robertson, 2006)Hist angiotensin converting enzymes argon small, basic proteins which bind to DNA, to form nucleoproteins - this helps the DNA to be packed tightly into coiled coils. Histones atomic number 18 robust in the amino acids arginine and lysine. Unlike most proteins, histones are water soluble. The functions of histones include packing nucleoproteins for compaction, and chromatin regulation. Histones abide modifications this set of modifications which are interdependent and responsible for controlling gene activity is called the Histone code. The histone code speculation of gene regulation was articulated by David Allis and colleagues. According to this theory, expression of certain regions of DNA is moody on and off by histone code. Histone proteins are one of the most highly conserved proteins in eukaryotes, emphasise their importance in cell biology.Heterochromatin is the condensed, dense form of chromatin, which is transcriptionally in quick hence it is peripherally located. It is abundantly seen in resting or reserve cells. It stains deeply during interphase (resting stage). The heterochromatin part of a chromosome either lacks genes or has repressed genes. Heterochromatin is seen in twain pro and eukaryotes. Euchromatin is delicate, thread-like, active form of chromatin. It is abundant in active, transcribing cells. The importance of euchromatin is because the portion of the DNA coil think for transcription must uncoil, to permit reading of the code. It stains lightly and its gene concentration is high. It is usually under active transcription and hence it is partially or fully uncoiled. Prokaryotes have only euchromatin. NUCLEAR LOCALISATION - lineament IN GENE EXPRESSIONThe vital facts about gene silencing are (Shak lai et al, 2007) 1. Chromosomes rich in genes are centrally located in the nucleus. Conversely, gene poor ones are located in the periphery.2. Inactive genes are silenced and translocated to the periphery. 3. If chromatin is moved to the periphery, it is silenced and rendered inactive. Gene silencing plays an important role in determining the spate of different cell lines during development and defining their temporal order. (Fischer, Merkenschlager 2002)cDNA MICROARRAY TECHNIQUEcDNA microarray (c -complimentary) is a light beam which helps us analyze and measure the activity of genes. This is done using the process of molecular hybridization. Adenine and tetraiodothyronine are complimentary Guanine and cytosine are complimentary. While studying two pieces of DNA, if they hybridize (bind to one another), then we can conclude they are complimentary strands - opposite strands of a single gene. Conversely, if they do not hybridize, then they are unrelated.Microarray expression techni que has many applications - Gene expression profiling (comparing gene expressions in normal and abnormal cells), study of multigenic diseases like Parkinsons disease,detection of